PURIFICATION OF HUMAN INTERFERON-? BY ANTIBODY AFFINITY CHROMATOGRAPY
- VernacularTitle:人?干扰素抗体亲和层析的研究
- Author:
Jiong DENG
- Publication Type:Journal Article
- Keywords:
human interferon;
purification;
antibody affinity chromatography
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Results of the establishment and application of anti-interferon antibody column are reported. Antiserum against human lymphoblastoid interferon (HuLyIFN) was obtained by immunizing sheep with partially purified Namalva interferon. After they were isolated from antiserum, the immunoglobulins against HuLylFN were adsorbed repeatedly on the "mock interferon"column and trypsin inhibitor column. Anti-interferon anti-body column was established by coupling the adsorbed anti-interferon antibody to Sepharose 4B. Crude Namalva interferon was purified by antibody affinity chro-matography in one step to a specific activity of 8?108 to 8?107units/mg protein with the recovery of over 100%. Peak of purified interferon activity in elution was seen mostly in fractions 1-3(3-8ml). Similar results were obtained in purification of human leukocyte interferon(HuLeIFN)by this column.The polyclonal antibody column purified recombined leukocyte interferon-?D (LrIFN-?D) to homogeneity as analyzed on SDS-polyacrylamide gel electrophoresis.
