Study of the Changes of Langerhans in Cultured Human Epidermal Cells
- VernacularTitle:人表皮细胞培养中Langerhans细胞变化的研究
- Author:
Shijun ZHANG
- Publication Type:Journal Article
- Keywords:
Culture of human Human Epidermal cells;
Langerans cells;
Mixed epidermal cell-lymphocyte cnlture response
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
It has been shown that epidermal Langerhans cells (LCs) were antigen presenting cells in cutaneous cell-mediated immuneresponse,and they were implicated in skin allograft rejection,If LCs can be depleted from skin grafts,the survival period of the allografts may be prolonged. Therefore,we observed the changes of LCs in cultured human epidermal cells (ECs) by ATPase staining,HLA-Dr monoclonal antibody indirect immunofluoresence and enzyme labelling avidin-biotin complex (ABC) staining.And the capacity of ECs to stimulate allogeneic lymphocyte proligeration before and after cultures was testedby mixed epidermal cell-lymphocyte culture response (MEcLR).We found that LCs in original skin explants were significantly decreased and deformed three days after culture.LCs were reduced more than 90 percent after 7 days of culture.LCs in original skin explants were approximately eliminated 14 days or more of culture.LCs were never seen within epidermal outgrowth in culture.The Ecs gained from the original skin explants which were cultured after 7 days and from epidermal outgrowth lost their ability to stimulate allogeneic lymphocyte transformation in MEcLR.These results indicate that LCs bearing HLA-Dr antigen in human are able to be depleted from epidermal grafts by epidermal cell culture,and suggest that the ECs cultured in our culture system can prolong their survival period as they are used as allografts.
