EFFECTS OF pOKEWEED MITOGEN AND ESCULENTOSIDE IN INDUCING IMMUNE INTERFERON FROM HUMAN SPLEEN CELLS
- VernacularTitle:两种商陆体外诱生免疫干扰素的作用
- Author:
Sikun YANG
- Publication Type:Journal Article
- Keywords:
immune interferon pokeweed mitogens esculentoside
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
The effects of pokeweed mitogen (PWM) and esculentoside (Es) in inducing immune interferon (IFN-?) from normal traumatic spleen and patient's spleen cells were studied.The interferon induced was proved to be human immune interferon (HUIFN-?) by determination of its sensitivity to pH 2 and unstabitity at 56℃ as well as neutraliztion by monoclonal antibody to HUIFN-?.The average titres of IFN-?,induced with PWM or Es,from the sphen cells of 10 traumatic pesons were 3.40?0.59 and 2.92?0.42 Log10 CPI50 U/ml respectively,and those from the spleen cells of 8 patients were 3.69?0.35 and 3.01?0.35 LogloCPI50 U/ml respectively.The effec of Es in inducing IFN-Y from either traumatic or patient's spleen cells was lower than that of PWM (P
