STUDIES ON THE EFFICACY OF DIFFERENT INDUCERS FOR INDUCING IMMUNE INTERFERON FROM HUMAN TONSILUR LYMPHOCYTES
- VernacularTitle:人扁桃体淋巴细胞诱生免疫干扰素的效用
- Author:
Zhigong XU
- Publication Type:Journal Article
- Keywords:
tonsillar cell mixed lymphocyte culture C.parvum
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
The efficacy of different inducers used to induce immune interferon (IFN-?)from ionsillar cells of onsillectomized normal children was sfudied.Tonsillar cells were made in suspension of 1?107 cells/ml treated with antibiotics,and cultured for 2-3 days and then supernants were assayed for IFN-r.The results show that cells of individual human tonsil could produce IFN-? spo ntaneously owing to the induction by pharyngeal normal flora.Esculentoside (Es) alone or .pokeweed mitogen (PWM) alone,their combinatian or with other inducers were capable of inducing IFN-?.Among them,PWM was more effective in inducing IFN-? than Es,and its efficacy was similar to that of PWM+ scoppolamine,but higher than that of PWM+C.parvum or PWM+Con A.Mixed lymphocyte culture (MLC) of human tonsils,with or without PWM or Es,could produce IFN-?,but slightly higher titer was obtained with MLC+PWM.The possible reasons for the difference of efficacy of various methods in inducing IFN-? from human tonsillar cells are discussed.
