A RAPID METHOD FOR DETERMINATION OF TRYPTOPHAN IN FOODS AND FEEDSTUFFS WITH FLUORIMETRIC METHOD
- VernacularTitle:荧光分光光度法快速测定食品和饲料蛋白质中的色氨酸
- Author:
Yuequn LIU
;
Jian HU
- Publication Type:Journal Article
- Keywords:
tryptophan food fluorimetry
- From:
Acta Nutrimenta Sinica
1956;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
A rapid method for directly determining tryptophan contents in foods and feedstuffs was described. l-5mg dry sample could be completely hydro-lyzed in a teflon tube containing 5N NaOH with 0.5% soluble starch under 145℃ for 4 hours in vacuum (760mmHg). The hydrolysate was then adjusted to neutral with 6N HCL in icebath and diluted by five times with 4M urea solution (pH = 11), and was measured at excitation wavelength 280nm and fluorescence wavelength 368nm. The sensitivity of the method was 5ng /10ml, and the recovery rate of 97-107% were obtained in the range of 1-5 ?g/10ml standard tryptophan. The value of a number of foods and feed-stuffs obtained were in agreement with the literatature values.
