Evaluation of the Influence of Thermal Injury on Bactericidal Capacity of Neutrophils through Studies on Changes of Cell Oxidative Metabolism
- VernacularTitle:从细胞的氧化代谢反应观察烧伤对中性粒细胞杀菌功能的影响
- Author:
Wenhua HUANG
- Publication Type:Journal Article
- Keywords:
Thermal injury;
Neutrophil;
Bactericidal function;
Oxidative metabolism;
Serum suppressive factors
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
Body defence functions are seriously impaired after thermal injury.Neutrophils,the important phagocytic cells against bacterial invasion,are also affected Therefore it is of value to study the functional statue of these cells in the postburn course.Two assays to observe the bactericidal function of neutrophils were adopted: the determination of the amount of H2O2 released and the126I taken by the activated neutrophils in oxidative metabolic processes.The results of the experiments on 2 animal models,inhalation injured dogs and scalded rats (TBSA 30% of third degree burns) with pseudomonas infection,showed that the cell oxidative metabolic response of the neutrophils was severely depressed in the first week postburn,indicating the presence of profound inhibition of the bactericidal function of the cells after thermal injury.In order to verify these results,direct yeast-killing assay was carried out and similar results were obtained.In order to explore the cause of the suppression of the bactericidal function,whether it was due to the presence of suppressive factors in the blood of burn victims,or due to the direct detrimental effect of thermal injury on the cells,further experiments to observe the crossed effects of burned serum on normal cells and normal serum on burned cells were performed.It was demonstrated that serum suppressive factors were present and the direct effects from thermal injury also played a part in suppressing the function
