THE ESTABLISHMENT OF THE NORMAL HUMAN FIBROBLAST CELL LINE AND A STUDY OF ITS BIOLOGICAL CHARACTERIZATION
- VernacularTitle:正常成人皮肤成纤维细胞系(NHF8)的建立及其生物学特性的研究
- Author:
Suming WANG
- Publication Type:Journal Article
- Keywords:
fibroblasts cell line diploid cell culture DNA repair
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
The normal human fibroblast cell line (NHF8) derivd from normal adult foreskin was passaged successfully in cell culture for more than 400 days.The cells were fibro-blast-like and were grown in the cell culture media NO.I with 15% newborn calf serum and were subcultured by trypsinization at a 1 : 2 split ratio. The cell line had a log phase doubling time of 26 h and its growth curve appeared in "s" shape. The mode of the chromosomes of the cell line was 46 and its frequency of sister-chroma -tid exchange (SCE), induced by UV irradiation or [not, was 0.143/per chromosome and 0.144/per chromosome respectively. The level of UV-induced unscheduled DNA synthesis (UDS) was also normal. The results demonstrate that the cell line is normal human diploid fibroblast cell line with normal function of excision repair of DNA damage and can be used as control cells in many studies.
