Establishment of Clq-ELISA for Detection of Antihuman Clq Monoclonal Antibodies
- VernacularTitle:抗人C1q单克隆抗体检测方法——C1q-ELISA的建立
- Author:
Peirong XIE
- Publication Type:Journal Article
- Keywords:
C1q;
Monoclonal antibodies;
ELISA
- From:Journal of Third Military Medical University
1984;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
The technique of Clq-ELISA has been established in this laboratory for the detection of antihuman Clq monoclonal antibodies(McAb). This technique, as verified by displacement test, blocking test, and cross titration test, possesses high specificity. It is more sensitive than double immunodiffusion as verified by the tests of 15 specimens of mouse antihuman Clq serum and has better reproducibility. It is concluded that this technique is very useful for the detection of anti-Clq McAb in the supernatant of hybridoma culture.
