COMPARISON OF RADIOIMMUNOASSAY (RIA) WITH ENZYMEIMMUNOASSAY (EIA) FOR MEASURING SERUM FERRITIN
- VernacularTitle:放射免疫法与酶联免疫法测定血清铁蛋白的比较
- Author:
Xiuyun LIU
- Publication Type:Journal Article
- Keywords:
radioimmunoassay enzymeimmunoassay serum ferritin
- From:
Acta Nutrimenta Sinica
1956;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Serum ferritin was recognized as a sensitive and reliable index for assessing body iron storage level. RIA and EIA for testing serum ferritin are now available. The aim of this study was to show the sensitivity and precision of RIA and EIA for testing serum ferritin and the relationship between the two assays. The result revealed that the two methods had sensitive-ties of 1 ng/ml and the CV% varied between 3.62 and 24.08 when the ferritin was assayed ten times on the same serum sample. The precision of two assays was similar, when RIA was compared with EIA on 35 serum ferritin determinations and the following linear regression equation was obtained:RIA = 0.82?EIA + 11.8, r = 0.98, n = 35The resulst of serum ferritin assayed with EIA were higher than that with RIA in general. It was considered that the EIA could offer a reliable alternative for the laboratories in which people do not prefer RIA for testing.
