ISOLATION AND PURIFICATON OF GLYCOPHORIN A FROM HUMAN ERYTHRO- CYTE MEMBRANE
- VernacularTitle:人红细胞膜血型糖蛋白A的分离与纯化
- Author:
Guanxiang JIANG
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
A method for isolation and purification of glycophorin A from human erythrocyte membrane was dercribed.The erythrocyte membrane was precipitated after haemolysed by distilled water and adding acetic acid, and laying overnight The precipitated membrane was treated with a hot phenol solution. After centriugation, the crude glycopro-teins were obtained in the supernatant.The crude glycoproteins were further purified by successive chromatographies on DEAE-Sephadex A-25 and Sephadex G-100.The purified glycophorin A appeared to be a single protein band on SDS-polyac-rylamide gel electrophoresis, stained with periodate-Schiff reagent.Its molecular weight was estmated to be about 60,000 by means of SDS-polyacrylamide gel electrophoresis.
