THE MEASUREMENT OF NEUTROPHIL FUNCTION AND OPSONIC ACTIVITY BY CHEMILUMINESCENCE
- VernacularTitle:中性白细胞功能和调理素活性的化学发光测定研究
- Author:
Yuanlin DONG
;
Zhiyong SHENG
- Publication Type:Journal Article
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A method for measurement of neutrophil function and opsonic activity by chemiluminescence (CL) was reported in this paper.The factors influencing the emission of CL were discussed. The experimental results suggested that CL depends on the concentration of luminol, the dilution of serum opsonin, the number of neutrophils and the environmental temperature. The optimum conditions defined in present study were: the concentration of luminol was 1?105M, the number of neutrophils 2?106/mml, the reactive temperature 37℃. According to the relationship between CL emission and the bactericidal activity of phagocytizing neutrophils, the measurement of CL may be used as a rapid, simple and usefull tool to determine the funetion of humoral-phagocyte axis.
