METHOD FOR THE DETERMINATION OF VITAMIN A IN BREAST MILK
- VernacularTitle:母奶中维生素A测定法
- Author:
Kezhi LIU
;
Jianwu XIE
- Publication Type:Journal Article
- Keywords:
breast milk vitamin A assay
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The ultraviolet spectrophotometric method for determination of vitamin A in breast milk was simplified. The milk sample was extracted directly with ammonia water, ethyl alcohol and ether, the saponification procedure be- ing omitted. The optimal height of the aluminum oxide adsorption column and the optimal rate of flow for elution were developed. The observation was made whether the elution for vitamen A was complete by identifying the fluorescence of vitamin A directly under ultraviolet light in the eluat-es collected instead of SbCl3-Vitamin A blue color reaction. The simultaneous estimation of Vitamin A in breast milk and in the standard solution of Vitamin A with this method showed E300nm/E325nm less than 73%, agreeable with the British Pharmacopoeia. The recovery was 100.8%. This procedure has advantages of high sensitivity, simplicity, time saving and economy.
