THE SIMULTANEOUS DETERMINATION OF RETINOL AND ALPHA-TOCOPHEROL IN PLASMA BY HIGH PRESSURE LIQUID CHROMATOGRAPHY
- VernacularTitle:高压液相色谱法同时测定血浆中视黄醇和?-生育酚
- Author:
Sufang ZHENG
;
Hui LI
;
Yuhai SUN
- Publication Type:Journal Article
- Keywords:
plasma retinol a-tocopherol high pressura liquid chromatography
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A microprocedure for the simultaneous determination of retinol and alpha-tocopherol in 100 ?l human plasma by reversed phase high pressure liquid chromatography without precolumn is detailed in this paper. A solvent system of methanol/water (96:4) provides clear separation of these two fat soluble vitamins. The chromatogram is complete in 12 min. The retinol and al-pha-tocopherol are quantitated by the peak height ratio and weight ratio method using retinyl acetate as an internal standard. The coefficient of variation of ten determinations is less than 5%. The mean recovery of four different concentrations of retinol and alpha-tocopherol are 102.2110.3% and 95.8?9.9% respectively. This microprocedure should be useful for assessment of the nutritional status of these two vitamins and might help to elucidate the relationship between them.
