PRELIMINARY STUDY OF A RADIOIMMUNOASSAY FOR DETECTION OF HBsAg
- VernacularTitle:固相放射免疫法检测乙型肝炎表面抗原的初步研究
- Author:
Lian ZHANG
;
Kangxian LUO
;
Ruhong LIN
- Publication Type:Journal Article
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
A solid-phase radioimmunoassay (RIA) method was developed for detection of HBsAg. Its reprodu-cibility. sensitivity and specificity were evaluated, and these results were compared with the Abbott kit (AUSRIA).Horse anti-HBs was used both for coating solid phase and iodination. Normal horse serum was applied in the labelled antiserum to prevent the non-specific reaction between different species of animal sera.The cutoff was 2.21? negative control value. The factor 2.21 was derived from the mean S/N value and its 3 SD of 553 normal sera, while the factor of AUSRIA was 2.1. The lowest detectable amount of HBsAg by our RIA method was 4. 1ng/ml, while that by AUSRIA 2. 1ng/ml, which was about 2 times more sensitive than our system.The prevalence of HBsAg in normal population detected by this RIA ranged from 12.9 to 17.7%. that in the patients with acute viral hepatitis was 60.6%. and in chronic viral hepatitis patients 89.9%.As hepatitis B virus infection is quite common in our country, it is important and urgent to use RIA widely to detect HBsAg carrier in the clinical diagnosis, selection of donors and epidemiological investigation.
