THE CONSTRUCTION OF HYBRID pBR322: RSF1010
- VernacularTitle:PBR322:RSF1010重组质粒的构建
- Author:
Zhaorong WEN
- Publication Type:Journal Article
- Keywords:
Plasmids cloning vector transformation, genetic recombination, genetic
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
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Abstract:
The plasmids pBR322 and RSF1010, extracted from E.coli HB802 and E. coli C600 respectively were treated with restrictive endonuclease EcoRI and ligated with T4 DNA ligase. C600 strains were then transformed with the hybrid plasmid, and thus 253 transformants were obtained.The figures of gel elctrophoresis and electromicrograph proved that PSMM1 was a pBR322 : RSF1010 composite hybrid.In E. coli C600 PSMM1 obtained resistance against AP, Tc and Sm.The orientation of pBR322 and RSF1010 in hybrid PSMM1 and the failure of introduction of this hybrid into P.Putida AC10 were discussed
