A STUDY OF DETERMINATION OF ASCORBIC ACID IN PLASMA BY FERRIC REDUCTION METHOD
- VernacularTitle:高铁还原法测定血浆维生素C的探讨
- Author:
Lanxing GAO
;
Guangyuan ZHANG
;
Jipeng LIU
;
Chinfan GU
- Publication Type:Journal Article
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The ascorbic acid in plasma was determined on the basis of reduction of ferric to ferrous ion and its formation of a colored complex with batho-phenanthroline. This method was highly correlated to Roe's dinitrophe-nylhydrazine method in the determination of plasma ascorbic acid of 16 normal adults and the correlation coefficient was 0.90. By animal experiment the new method may be used to discriminate different levels of plasma ascorbic acid. It is rather simple and water bath is not required. Only 0.25ml of plasma is needed for each determination. So this method is useful in assessing the ascorbic acid nutriture in tield survey.