STUDIES ON THE PURIFICATION OF LEUKOCYTE INTERFERON FROM HUMAN UMBILICAL CORD BLOOD
- VernacularTitle:人脐血白细胞干扰素纯化研究
- Author:
Zhongtian QI
;
Ping DU
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The purification system described in this paper includes two steps. The first step may be called "rough purification" by using potassium thiocyanate (KCNS) -alcohol fractionation precipitation method. The second step is "fine purification" by using Blue Dextran-sepharose 4B (BDS) column affinity chromatography methodThe experimental results showed that the optimum condition for precipitating the interferon (IFN) from the crude IFN solution could be made by adding 0.5M KCNS, and then adjusting pH to 4.0. The optimum pH values in separating out the IFN from acid alcohol-interferon solution were 5.5 to 7.2. The results between the salting out once and twice by KCNS were not significantly different. Centrifugation with centrifuge maintaining lower temperature with cold alcohol could be used instead of refrigerated centrifuge during purification. The IFN can be purified to about 106 international units per mg of protein with about 70% recovery on the first step of purification. In the IFN yielded on the second step of purification, the maximum specific activity of IFN in the collecting fractions was increased up to about 107 international units per mg of protein with a recovery rate of over 40%.The present experiments may offer the possibility in purifying the IFN for clinical use. The Suggested purification method will also render some valuable reference to purify other types of IFN.
