A STUDY ON THE THIAMINE REQUIREMENT OF CANTON MIDDLE SCHOOL BOYS
- VernacularTitle:广州市中学男生硫胺素需要量的研究
- Author:
Pengcheng HSU
;
Huilien KUAN
;
Yunhsian WU
;
Lanchen KUNG
- Publication Type:Journal Article
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
This study is undertaken simultaneously with the experiments for the determination of riboflavin requirement of middle school boys in Canton. The same subjects serve in both experiments. When the loading tests are performed, 2.0 mg of thiamine is given orally at the same time the riboflavin is administered. Supplements of 0.5, 1.0 and 1.5 mg thiamine are given at the same time and in the same order as the ribofiavin. The same urine samples are analyzed for thiamine as well as riboflavin. Thiamine is analyzed by the thiochrome method of Consolazio.The thiamine content in the one-hour urine samples of the subjects before the supplement averages 5.4 to 8.9 micrograms for the 4 groups. After supplementation, the thiamine content of the fasting samples of 1.0 and 1.5 mg supplement groups show definite increase, while the other two groups remain near the same levels.After the loading test, the 4-hour total excretion amounts to about 5 per cent of the 2 mg taken, with almost no difference among the groups. When the intake of thiamine has been supplemented for two weeks, the response to the loading test varies. The 0.5 mg supplemented group excreted 4.35% of 2 mg in 4 hours, while the 1.0 and the 1.5 mg groups excreted about 8%. The hourly excretions are shown in Fig. 1. The peak of excretion of the 1.5 mg supplemented group changes from the second to the first hour after the load test. The thiamine intake of these subjects is estimated to be about 1 mg daily. They are free from symptoms associated with thiamine deficiency and their 1-hour urine samples show that the quantity excreted may be within the range considered normal for healthy subjects, yet supplementation of 1.0 or 1.5 mg of thiamine is able to cause a greater and faster urinary excretion of thiamine. An additional intake of at least 1.0-1.5 mg thiamine to their ordinary dietary intake of about 1 mg may be desirable to ensure an optimum status of thiamine nutrition in the middle school boys.
