Preparation and characterization of anti-human AchR monoclonal antibodies made from rat bone marrow
- VernacularTitle:分泌抗人乙酰胆碱受体抗体骨髓杂交瘤细胞的制备及鉴定
- Author:
Qing ZHAO
;
Na YIN
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
AchR;
Bone marrow;
Hybridoma;
Myasthenia gravis
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare anti-AchR monoclonal antibodies from rat bone marrow.Methods:The extracellular region of human AchR ?1 subunit was expressed in E.coli and purified using a Ni-NTA column.Then the protein was refolded by dialyzing.Lewis rats were immunized with purified AchR ?1 subunit and B cells from bone marrow and spleen were fused with myeloma cells to generate monoclonal antibodies.Results:Human AchR ?1 subunit was successfully expressed in E.coli.Rats immunized with the refolded protein were shown symptoms of Myasthenia gravis.Hybridoma cells were produced using B cells from rat bone marrow and spleen.Conclusion:Bone marrow cells can be immortalized using hybridoma method,which can be used for further study of Myasthenia gravis.
