Effects of ?-lipoic acid on bone metabolism in lead-poisoned juvenile rats
- VernacularTitle:?-硫辛酸对染铅幼年大鼠骨代谢的影响
- Author:
Dan PEI
;
Deyu ZHENG
;
Wei SHAN
;
Shujian QIN
- Publication Type:Journal Article
- Keywords:
?-lipoic acid;
lead;
bone;
osteocalcin(OC);
alkaline phosphatase(ALP)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of ?-lipoic acid on bone metabolism in lead-poisoned rats.Methods Totally 31 SD rats were randomly divided into two groups,8 rats in blank control group and 23 in lead group that received gastric lavage with lead acetate(230 mg/L).After 40 days,3 rats from each group were taken for the measurement of lead in the blood and bone.The rats in lead group were then randomly divided into four groups: lead control group and three lead groups with different concentrations of ?-lipoic acid [30,60 and 100 mg/(kg?d)],with 5 rats in each.At the end of the experiment(80 d),the rats were killed and the samples of whole blood,serum and bone were collected to detect osteocalcin content with radioimmunoassay and alkaline phosphatase expression with immunohistochemistry staining.Results ① Blood and bone lead contents in each ?-lipoic treatment group were significantly lower than those in lead control group(P
