Enhancement of photodynamic therapy sensitivity by cisplatin in human cervical carcinoma cell lines
- VernacularTitle:小剂量顺铂增加人宫颈癌Hela和Siha细胞对光动力治疗敏感性的研究
- Author:
Haiyan WANG
;
Youzhong ZHANG
;
Kan JIANG
;
Hui JIAO
;
Zhaojing WANG
- Publication Type:Journal Article
- Keywords:
5-aminolevulinic acid;
cisplatin;
photodynamic therapy;
immunohistochemistry;
cervical carcinoma cell line
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of small-dose cisplatin in enhancing photodynamic therapy sensitivity and apoptosis in human cervical carcinoma cell lines.Methods The Hela and Siha cells were divided into four groups(blank control group,photodynamic therapy only,cisplatin only,photodynamic therapy and cisplatin).The effects on proliferation of Hela and Siha cells in the four groups were examined by MTT assay.The expression of P185c-erbB-2 in Hela and Siha cells affected was detected by immunocytochemistry.Results The degree of apoptosis caused by the joint application of cisplatin and photodynamic therapy was greater than that caused by cisplatin or photodynamic therapy alone.The expression of P185c-erbB-2 in cells declined correspondingly.However,the order of cisplatin and photodynamic therapy did not cause obvious differences in the effect.Conclusion The in vitro proliferation of Hela and Siha cells is restrained obviously by cisplatin combined with photodynamic therapy;the effect is greater than that of cisplatin or photodynamic therapy only.The mechanism of cispatin's enhancement of photodynamic therapy sensitivity may be related to inhibiting P185c-erbB-2 expression.
