Effect of statins on ABCA1 gene expression in neurons and astrocytes
- VernacularTitle:他汀类药物对神经细胞和神经胶质细胞ABCA1基因表达的影响
- Author:
Huilin GONG
;
Kuo LI
;
Guanjun ZHANG
;
Huilian HOU
;
Weijiang DONG
- Publication Type:Journal Article
- Keywords:
ATP-binding cassette transporter 1(ABCA1);
simvastatin;
pravastatin
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of statins on ATP-binding cassette transporter 1(ABCA1) gene expression in neurons and astrocytes.Methods Primary human astrocytes and neuron cell line HCN-2 were incubated with simvastatin and pravastatin at different concentrations and under different conditions.Using RNA isolated from the cultured cells,we performed quantitative PCR to measure the ABCA1 gene expression in astrocytes and neurons.The protein expression of ABCA1 was measured by Western blot.Results The two statins significantly decreased the ABCA1 gene and protein expressions.Compared with pravastatin,simvastatin significantly reduced the expression of ABCA1 in neurons and astrocytes by 95% and 75%,respectively(both P
