Preparation and standardization of paratyphi a specific transfer factor
- VernacularTitle:甲型副伤寒特异性转移因子的制备和检定
- Author:
Jianwei ZHOU
;
Junmin LUO
;
Wanbang SUN
;
Rui MA
;
Dan TIAN
- Publication Type:Journal Article
- Keywords:
paratyphi a;
specific transfer factor;
preparation;
standardization
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a new kind of biological agent initially,which will be used for emergent prevention or adjuvant therapy for paratyphia.Methods Paratyphia specific factor(PA-STF) was prepared in vivo and scanned with multi-wavelength using ultraviolet spectrophotometer.Then we determined the content of polypeptide and ribose with orcinol assay and modified Lowry assay respectively,followed by sterility test,pyrogen test and safety test.The immunological activity was assayed by immune protection test.Results The physico-chemical properties of PA-STF accorded with the criteria of Chinese Bioproduct Rules(2000 edition).In the immune protection test,the survival rate of mice was higher in the two experiment groups than in control group and NS group(P
