Regulation on function and maturation of plasmacytoid dendritic cells by Astragalus polysaccharide
- VernacularTitle:黄芪多糖对浆细胞样树突状细胞功能及成熟的影响
- Author:
Limin LIU
;
Yanming ZHANG
;
Shuhua LU
;
Yumei SUN
;
Guangsheng HE
- Publication Type:Journal Article
- Keywords:
Astragalus polysaccharide;
Plasmacytoid dendritic cells;
Maturation
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism for immunomodulatory activity of Astragalus polysaccharide (APS) with peripheral blood mononuclear cells(PBMC)-derived plasmacytoid dendritic cells (pDCs) from healthy volunteers.Methods:Healthy volunteers-derived pDCs were sorted by flow cytometry,then incubated with APS (0m50,100,200 mg/L ).After 24 hours,the concentration of IFN-?,TNF-?,IL-6 was detected using ELISA.After 5 days,the cultured cells were collected and analyzed by flow cytometry,light microscope and electron microscope scanning.Results:APS-treated pDCs secreted higher level of IFN-?,TNF-?,IL-6.APS could promote differentiation of pDCs to dendritic cells (DCs) which displayed more matured morphology and immunophenotypes compared to the untreated-pDCs.Conclusion:APS could increase the immune function of pDCs,promote differentiation and maturation of pDCs.
