Bioinformatics analysis of OLFM1 epitope and preparation of polyclonal antibody
- VernacularTitle:OLFM1蛋白的生物信息学分析及其多克隆抗体制备与鉴定
- Author:
Xiaoying WANG
;
Peige DU
;
Chunfeng WU
;
Xiao HAN
- Publication Type:Journal Article
- Keywords:
OLFM1;
Bioinformatics;
Polyclonal antibody
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze OLFM1 by bioinformatics and prepare its polyclonal antibody.Methods:According to the bioinformatics analysis and prediction of the possible high structure,hydrophobicity and antigenicity of OLFM1 and the principal of antibody project,a partial peptide of OLFM1 with 20-amino acid residues with was synthesized after homology search.The synthesized peptide was then used to immunize rabbit.The specificity and titer of polyclonal antibody against OLFM1 were identified via ELISA and Western blot.Results:Polyclonal antibody against OLFM1 was proved to recognize OLFM1 protein specifically,and its titers reached 1?32 000.Conclusion:By the bioinformatics analysis and prediction,the hydrophilicity and antigenicity of OLFM1 are analyzed.The polyclonal antibody against OLFM1 is successfully obtained.
