Proportional influence of Th1/Th2 lymphocytes after transferring the recombinant of PcDNA3.0/p27~(kip1) eukaryotic expressing plasmid into asthma model of mice
- VernacularTitle:PcDNA3.0/p27~(kip1)真核表达质粒对小鼠哮喘模型Th1/Th2的影响
- Author:
Yang SUN
;
Ping CHEN
;
Haitao ZHAO
;
Li SUN
- Publication Type:Journal Article
- Keywords:
p27;
Gene therapy;
Immune regulation;
Asthma
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of the proportional imbalance of Th1/Th2 in allergic asthma model by transferring the recombinant of PcDNA3.0/p27kip1 eukaryotic expressing plasmid into allergic asthma model.Methods:KM mice were distributed into three groups:healthy control mice(group A);Ovalbumin(OVA)-sensitized/challenged mice(group B) and OVA-sensitized/challenged mice intratracheally administered with PcDNA3.0/p27kip1 plasmid(plasmid-treated mice,group C).KM mice were sensitized and challenged intratracheally with OVA,then transfected with the recombinant PcDNA3.0/p27kip1 eukaryotic expressing plasmid which was constructed by our laboratory crew through vein injection.The p27 protein expression in lungs 36 h after transfection was analyzed by Western blot;Flow cytometry was used to analyze the proportion of CD4 (+)and CD8(+) T lymphocyte subsets in the spleen.The serum levels of IL-4,IL-5,IL-13 and IFN-? were measured by ELISA.Results:Compared to the group A,the expression of p27 protein was significantly reduced,and the proportion of CD4(+)lymphocytes and serum levels of IL-4,IL-5 and IL-13 were significantly higher but CD8(+)lymphocytes and IFN-? was significantly decreased in group B.However,in group C,the expression of p27 protein was significantly increased;Whereas the proportion of CD4 (+) lymphocytes,serum levels of IL-4,IL-5 and IL-13 were significantly decreased.CD8 (+) lymphocytes and IFN-? were significantly increased than those in group B.Conclusion:p27 could be expressed in lungs of plasmid-treated mice and regulate the proportion of Th1/Th2 subset by means of adjusting cell cycles.This method may serve as a potential therapeutic option for the treatment of asthma.
