Expression of green fluorescent protein gene in rat chondrocytes mediated by lentiviral vector
- VernacularTitle:慢病毒介导绿色荧光蛋白基因转染大鼠软骨细胞表达的研究
- Author:
Wenxiong CHEN
;
Xiaohong YANG
;
Siming LI
- Publication Type:Journal Article
- Keywords:
lentiviral vector,green fluorescent protein, chondrocytes
- From:
Orthopedic Journal of China
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the transfection and expression of green fluorescent protein gene in rat chondrocytes by constructing a combination lentiviral vector containing green fluorescent protein gene.[Method]293T cells were co-transfected with the vector plasmids pLentiLox 3.7,the packaging plasmids pRSV-REV,pMDL g/p RRE and the envelope plasmids VSV-G to produce lentiviral vector particles.Rat chondrocytes were isolated,cultured and infected by the lentiviral vector particles.The expression of reporter gene GFP was observed under fluorescent microscope.[Result]At 96 hours after transfection,a strong expression of GFP could be found in 293T cells.The viral titer was 3.0?103ifu/?l.GFP expression was observed in about 80% of the rat chondrocytes.[Conclusion]A combination lentiviral vector containing green fluorescent protein gene was successfully established and it could infect rat chondrocytes efficiently,which will provide a basis for gene modification of chondrocytes through RNA interference.
