Biological characteristics of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells and their differentiation into Schwann-like cells
- VernacularTitle:人脐带Wharton胶间充质干细胞的生物学特性及雪旺细胞分化研究
- Author:
Yu WANG
;
Li ZHANG
;
Jiang PENG
- Publication Type:Journal Article
- Keywords:
umbilical cord, mesenchymal stem cells, Schwann cell
- From:
Orthopedic Journal of China
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the biological characteristics of Wharton’[KG-38x]s jelly-derived mesenchymal stem cells (WJMSCs) and their differentiation into Schwann cell-like cells, and to provide a new cell seed source for nerve tissue engineering or cell therapy. [Methods]Umbilical arteries, veins and umbilical cord tunica externa were removed , and the remaining tissue (Wharton’[KG-38x]s jelly) was harvested.The umbilical cord was cut into small fragments and cultured with DMEM in orderto gather. Cultured WJMSCs were confirmed by the detection of MSC-specific cell-surface markers. WJMSCs were differentiated along a glial cell lineage using an established cocktail of growth factors. Immunocytochemical staining and Western blot were used to evaluate the characteristics of differentiated WJMSCs.[Results]WJMSCs were immunonegative for the haemopoietic cell-surface markers (CD34 and CD45) and neural stem cell marker (nestin), and immunopositive for MSC surface markers CD44, CD105, Stro-1 and vimentin. WJMSCs treated with a mixture of glial growth factors adopted a spindle-like morphology similar to Schwann cells. Immunocytochemical staining and Western blot analysis revealed that the treated cells expressed the glial markers p75 and glial fibrillary acidic protein indicative of differentiation.[Conclusion]WJMSCs can be differentiated into the cells that are Schwann-like in terms of morphologic features and phenotype and may be suitable as Schwann-cell substitutes for nerve repair in clinical application.
