Protective effect of felodipine on human umbilical vein endothelial cells injured by oxidized low-density lipoprotein
- VernacularTitle:非洛地平对氧化损伤的人脐静脉内皮细胞的保护作用
- Author:
Jie QI
;
Ying SHENG
;
Jianbao ZHENG
;
Zuyi YUAN
- Publication Type:Journal Article
- Keywords:
felodipine;
oxidized low-density lipoprotein (ox-LDL);
oxidative stress;
atherosclerosis;
human umbilical vein endothelial cell;
intercellular adhesion molecule-1 (ICAM-1);
vascular cell adhesion molecule-1 (VCAM-1)
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of felodipine on reactive oxygen species (ROS) generation,mRNA level of inflammatory factors such as intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1),in human umbilical vein endothelial cells (HUVECs) injured by oxidized low-density lipoprotein (ox-LDL) so as to explore felodipine's anti-atherosclerosis mechanism independent of its anti-hypertensive effect. Methods Isolated HUVECs were treated with ox-LDL at different concentrations (6,12.5 and 25 mg/L) for 24 hours so that the optimal concentration and time of ox-LDL treatment were selected. Then the cells were incubated with ox-LDL and treated with felodipine at different concentrations (0.1,1 and 10 ?mol/L). Intracellular ROS level was determined by flow cytometry (FCM). Expressions of inflammatory factors ICAM-1 and VCAM-1 were measured by real time-polymerase chain reaction (real time-PCR). Results ROS generation was increased in HUVECs after treatment with different concentrations (6,12.5 and 25 mg/L) of ox-LDL for 24 hours and there was a significant difference at 25 mg/L ox-LDL (P
