Irbesartan inhibits high glucose-induced lipid oxidation effect and human umbilical vein endothelial cell injury
- VernacularTitle:厄贝沙坦抑制高浓度葡萄糖诱导的脂质氧化效应和人脐静脉血管内皮细胞损伤
- Author:
Min LU
;
Qun LU
;
Yan REN
;
Gang TIAN
- Publication Type:Journal Article
- Keywords:
irbesartan;
glucose;
low-density lipoprotein;
oxidative stress;
atherosclerosis
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the influences of irbesartan on high glucose-induced lipid oxidation effect and human umbilical vein endothelial cell (HUVEC) injury. Methods HUVECs of the logarithmic phase were divided into four groups:normal-concentration glucose control group (glucose final concentration of 5.5 mmol/L),high-glucose group (glucose final concentration of 33.3 mmol/L),irbesartan intervention group,and anti-oxidant N-acetyl-cysteinyl acid (NAC) intervention group. The intervention groups were first treated with irbesartan (10-5mmol/L) and NAC (10 mmol/L) for 1 h,respectively,then co-incubated with high glucose (glucose final concentration of 33.3 mmol/L) for 24 h,48 h and 72 h. Malondialdehyde (MDA) content and superoxide dismutase (SOD) activity of culture supernatant were determined by TBARS method and spectrophotometric assay,respectively. Results MDA content increased significantly (P0.05). Compared with that in high glucose group,MDA content significantly decreased (P
