In vitro effect of celecoxib on osteoblast apoptosis induced by titanium particle in neonatal rats
- VernacularTitle:噻莱昔布对体外钛微粒诱导大鼠成骨细胞凋亡的影响
- Author:
Eryou FENG
;
Yiyuan ZHANG
;
Yu LIN
- Publication Type:Journal Article
- Keywords:
celecoxib, COX-2, osteoblast, aseptic loosening
- From:
Orthopedic Journal of China
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To study the effect of celecoxib on osteoblast apoptosis induced by titanium particle in neonatal rats. [Methods]The best in vitro celecoxib serum concentration for promoting proliferation of osteoblast in neonatal rats was obtained through MTT.One group of rat osteoblast in vitro was cultured with serum containing saline and titanium particle and the other was cultured with serum containing celecoxib and titanium particles.Concentrations of TNF-? and PGE-2 in cells collected after 24 hours were analyzed by ELLSIA,COX-2 mRNA and protein were indicated by FO-PCR and Westren blot.[Results]Some cellar factors such as COX-2,TNF-? and PGE-2 could be induced by titanium particle in in vitro,but celecoxib could inhibit expression of these factors by COX-2 pathway.[Conclusion]Celecoxib coule be a promising drug for treatment of aseptic loosening.
