Histone deacetylase inhibitor in inhibiting proliferation and inducing differentiation of human osteogenic sarcoma cells
- VernacularTitle:组蛋白去乙酰化酶抑制剂对人骨肉瘤细胞抑制增殖和诱导分化的研究
- Author:
Fan DING
;
Zengwu SHAO
- Publication Type:Journal Article
- Keywords:
osteosarcoma, histone deacetylase, trichostain A, differentiation
- From:
Orthopedic Journal of China
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To study the effect of histone deacetylase inhibitor-Trichostatin A(TSA) on proliferation and differentiation of human osteogenic sarcoma cell. [Methods]Human osteogenic sarcoma cell line MG-63 was treated in vitro with various concentrations of TSA, a potent and specific histone deacetylase inhibitor. Proliferation suppression was observed by MTT method and inverted microcopy before and after TSA treatment, and the cell growth curve was obtained. The cell growth and invasion ability were measured with the colony-formation rate in soft agar test. Flow cytometry was used to investigate the cell cycle.[Results]TSA significantly inhibited the proliferation of the human osteogenic sarcoma cell in a dose-dependent and time-dependent manner. The cell experienced benigh morphological differentiation. The colony-formation rate in semi-solid agar was significantly decreased(P
