Effects of ouabain on Na~+-K~+-ATPase activity and the expression of dopamine D_1 receptor in rat kidney cortex
- VernacularTitle:哇巴因对大鼠肾皮质Na~+-K~+-ATP酶活性及多巴胺D_1受体mRNA表达的影响
- Author:
Yurong ZHANG
;
Zuyi YUAN
;
Yanping REN
- Publication Type:Journal Article
- Keywords:
Na+-K+-ATPase activity;
dopamine D1 receptor;
ouabain;
hypertension;
sodium handling
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of chronic ouabain treatment on Na+-K+-ATPase activity and the expression of dopamine D1 receptor in rat kidney cortex. Methods A total of 28 male Sprague-Dawley (SD) rats were randomly divided into ouabain group and control group,which were treated with ouabain or saline for 5 weeks; rat tail systolic blood pressure (SBP) was recorded weekly. Rats were sacrificed after 3 and 5 weeks,respectively. Then Na+-K+-ATPase activity and the expression of dopamine D1 receptor in rat kidney cortex were measured by colorimetric assay and real-time PCR,respectively. Results After 3 weeks of ouabain treatment,the mean SBP did not change significantly,but the Na+-K+-ATPase activity increased (P
