Inhibiting and apoptosis-inducing effects of recombinant Ganoderma Lucidum immunoregulatory protein on HL60 cells
- VernacularTitle:重组灵芝免疫调节蛋白抑制HL60细胞增殖及诱导凋亡的研究
- Author:
Qi GUO
;
Han SUN
;
Chongyang LIANG
;
Shuqin ZHANG
;
Zhiyi LIU
;
Fei SUN
- Publication Type:Journal Article
- Keywords:
Ganoderma Lucidum;
Immunoregulatory protein;
Apoptosis
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibiting and apoptosis-effects inducing of the recombinant Ganoderma Lucidum immunoregulatory protein(rLZ-8) on HL60 cells.Methods:HL60 cells were cultured and the inhibited rate of HL60 cells was measured by means of MTT assay.Apoptosis rate was detected with annexinV/PI.The Calcium ion level was analyzed by Fluo-3/AM stain.Caspase-3 activity was assessed by caspase-3 colorimetric assay.Results:The experiments indicated that rLZ-8 could induce the HL60 cells apoptosis.The calcium ion level was increased and caspase-3 activity elevated in HL60 cells after treated by rLZ-8.Conclusion:rLZ-8 could inhibit the proliferation and induce apoptosis of HL60 cells efficiently.The mechanism may be related to increased calcium ion level and elevation of caspase-3 activity.
