Preparation and identification of hPPAR?2 monoclonal antibody
- VernacularTitle:hPPAR?2单克隆抗体的制备与鉴定
- Author:
Yan LI
;
Zhiyong HAO
;
Xiaoguang CHEN
- Publication Type:Journal Article
- Keywords:
hPPAR?2;
Monoclonal antibody;
Prepare;
Specificity
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare and identify the monoclonal antibody against hPPAR?2.Methods:cDNA of hPPAR?2 was obtained from pMD18-T/hPPAR?2.hPPAR?2 was expressed in prokaryocyte and purified successfully.The anti-hPPAR?2 monoclonal antibodies (mAbs) 1B4,3H2,3H10,10D6 and 10D8 were produced by immunization with purified hPPAR?2.The specificity of the antibodies was identified by ELISA,Western blot assay and immunocytochemistry.Results:Five novel murine monoclonal antibodies only against hPPAR?2 were harvested,and they were all specific to hPPAR?2.Conclusion:The antibodies obtained provide more useful tools for following research and settling the basis for screening new drugs and mechanism.
