Study on fast purification of monoclonal antibody against rhTF_(243) protein with the technique of HCIC
- VernacularTitle:一种利用HCIC快速纯化抗rhTF_(243)单克隆抗体方法的研究
- Author:
Fengmei ZHAO
;
Yanhong QI
;
Yongji HE
;
Weiwei PAN
;
Xiaoxia LU
;
Yali LIANG
;
Yi ZHAO
- Publication Type:Journal Article
- Keywords:
HCIC;
Affinity chromatography;
Monoclonal antibody;
Mouse ascites;
purification
- From:
Chinese Journal of Immunology
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a two-step method for purification of monoclonal antibody rhTF243 protein from mouse ascites by using hydrophobic charge induction chromatography(HCIC) and affinity chromatography with protein A sepharose CL-4B.Methods:The ascites was first purified by HCIC after centrifugation and filtration.Then the fraction containing the protein of interest was directly purified by affinity chromatography with protein A sepharose CL-4B.Results:The purity of the obtained monoclonal antibody was up to 97% with recovery of 73% and of high activity.Conclusion:The method for purification of monoclonal antibody is developed using HCIC and Protein A affinity chromatography and the obtained antibodies are of high purity and activity.
