Effects of Wnt/?-catenin signal pathway in proliferation of Schwann cells promoted by pyrroloquinoline quinine
- VernacularTitle:Wnt/?-catenin信号通路在吡咯喹啉醌促雪旺细胞增殖中的作用研究
- Author:
Bin HE
;
Shiqing LIU
;
Haohuan LI
- Publication Type:Journal Article
- Keywords:
pyrroloquinoline quinone,Schwann cell,Wnt/?-catenin,proliferation
- From:
Orthopedic Journal of China
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the effects of Wnt/?-catenin signal pathway on Schwann cells proliferation promoted by pyrroloquinoline quinine (PQQ) and its molecular mechanisms.[Method]Schwann cells were cultured and purified in vitro. The purity was identified by S-100. PQQ of 10 nmol/L and 100 nmol/L were added into culture medium for 24 hours,respectively. Then the morphological changes promoted by PQQ were observed by inverted microscope. The expression of ?-catenin was detected by RT-PCR and Western blot in Schwann cells promoted by PQQ of different concentration for 72 hours.[Result]Morphological change was observed in Schwann cells treated by PQQ of 10 nmol/L and 100 nmol/L. The most obvious morphological changes took place in the Schwann cells treated by 100 nmol/L of PQQ,the RT-PCR and Western blot results showed that PQQ of 1-1000 nmol/L could up-regulate the expression of ?-catenin,especially when Schwann cells was treated by PQQ of 100 nmol/L(P
