Effect and mechanism of ischemic postconditioning on lung injury induced by ischemia-reperfusion of skeletal muscle in the hind limbs of rats
- VernacularTitle:缺血后处理对骨骼肌缺血再灌注后肺损伤保护作用的机制
- Author:
Hui CAO
;
Xinhua HU
;
Jiaan HE
;
Qiang ZHANG
;
Shijie XIN
- Publication Type:Journal Article
- Keywords:
Ischemic postconditioning?Reperfusion injury?Muscle,skeletal?Lung injury?Rats,Wistar
- From:
Chinese Journal of Current Advances in General Surgery
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of ischemic postconditioning(I-postC)on the lung injury following ischemia-reperfusion(I/R)of skeletal muscle in the hind limbs of rats.Methods:The rat model of hind limbs I/R injury was established by subrenal abdominal aorta cross-clamping for 4 hours.Forty-eight rats were divided into 3 groups:I/R group,IPC and I-postC group.Each group received 4 hours of ischemia and then 12 or 24 hours of reperfusion respectively.The tissue morphology,wet-to-dry weight(W/D)ratio,malondialdehyde(MDA)and myeloperoxidase(MPO) of lung tissue were compared.The expression of ICAM-1 mRNA in lung was also studied by RT-PCR or in situ hybridization.The protein product was detected by Western blot.Results:In IPC and I-postC groups,all parameters decreased significantly compared with I/R ischemia group(P
