Protective effect of ?-scretase substrate-base peptide on cellular model of Alzheimer's disease
- VernacularTitle:?-分泌酶底物肽对阿尔茨海默病细胞模型的保护作用
- Author:
Yuanyuan CUI
;
Shehui SHI
;
Haitao HU
;
Weijiang DONG
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease(AD);
?-scretase substrate-base peptide(BACEsp);
cellular model of AD
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of ?-scretase substrate-base peptide(BACEsp) on cellular model of Alzheimer's disease(AD).Methods The cellular model of AD established with neuroblastoma cell SK-N-SH induced by amyloid precursor protein(APP) was infected with the supernatants including recombinant retrovirus pLXSN-BACEsp.MTT assay was used to test cell viability and immunocytochemistry was used to observe APP expresion in cells before and after BACEsp treatment.Results Cell viability and APP expression in the cells that had been first infected by recombinant retrovirus pLXSN-BACEsp were apparently higher than those only transfected by APP and last infected by recombinant retrovirus pLXSN-BACEsp.Conclusion BACEsp has an obviously protective effect on APP-induced neuron toxicity of cellular model of AD.
