Effects of TGF-?1 on production of a-SMA and extracellular matrix in flexor tendon sheath fibroblasts
- VernacularTitle:TGF-?1对腱鞘成纤维细胞a-SMA及细胞外基质合成的影响
- Author:
Dapeng JIANG
;
Zhaozhu LI
;
Yubo ZHANG
- Publication Type:Journal Article
- Keywords:
transforming growth factor-?,flexor tendon,fibroblast,a-smooth muscle actin,collagen
- From:
Orthopedic Journal of China
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To examine the effects of TGF-?1 on the production of ?-SMA and extracellular matrix in flexor tendon sheath fibroblasts. [Methods]Sheath fibroblasts were obtained from rabbit flexor tendons.Cell culture was supplemented with 5ng/ml of TGF-?1.After 48 hours incubation,the production of a-SMA was assayed by Western-Blot.The productions of collagen I and fibronectin in supernatants culture were examined using ELISA.[Results]Treatment with TGF-?1 significantly stimulated a-SMA production in flexor tendon sheath fibroblasts (P
