Comparison of Three Methods to Determine Cholesterol with High Density Lipoprotein
- VernacularTitle:三种高密度脂蛋白胆固醇测定方法的比较
- Author:
Mingjing WEI
- Publication Type:Journal Article
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
Three methods to determine HDL-Ch, polyethylene glycol precipitation, he-parin-Mn*, and sodium phosphotungstate-magnesium, were compared. It was found that when 20 g/dl (20%) PEG-6000 was mixed with equal volume of serum and the mixture was centrifuged, well seperation of HDL-Ch could be obtained even the serum triglyceride level in a sample was as high as 1000mg/dl. Since this method is easy to manipulate and the reagent is stable, it is suitable to be used in hospital labs as aroutine procedure.