Determination for Total Flavonoids in Health Food with the Ultrasonic Extraction Technology
- VernacularTitle:保健食品中总黄酮的超声波提取条件优化研究
- Author:
Jian WU
;
Xingbi DAI
;
Xiao XIAO
- Publication Type:Journal Article
- Keywords:
Spectrophotometry;
Flavonoids;
Ultrasonic extraction;
Orthogonal test design
- From:
Journal of Environment and Health
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
extraction time.The extraction of total flavonoids in health food was the highest under the situations of ethanol concentration 60%,solid-to-liquid ratio 1:20 and ultrasonic time 20 min.The average value was 5.370 mg.The linear range of total flavonoids was between 1.0-40 ?g/ml while the regression equation is y=0.003 3x-0.010 9,correlation coefficient was 0.999 5 and detection limit was 1.0 ?g/ml.The standard addition recovery rates of this method were 95%-104%(n=3),RSD was 1.717%(n=5).Conclusion Under the optimum situation,this method has high precision and accuracy while the blank is low and stable as well.It is applicable to the detection of total flavonoids in health food and some other foods.
