Animal experimental study on incorporation of silk fibroin reducing the risk of cardiovascular complication during vertebroplasty
- VernacularTitle:丝素蛋白/磷酸钙骨水泥降低椎体成形术中心血管并发症风险的实验研究
- Author:
Tao DING
;
Huilin YANG
;
Ruisheng XU
- Publication Type:Journal Article
- Keywords:
vertebroplasty,silk fibroin,calcium phosphate cement,pulmonary embolism,pig,animal model
- From:
Orthopedic Journal of China
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To develop an animal pulmonary cement embolism model,by which the effect of polymethylmethacrylate(PMMA),calcium phosphate cement(CPC) and silk fibroin/calcium phosphate cement composite(SF/CPC) on the hemodynamic change,respiratory function and the antithrombin activity were measured after the pulmonary embolism caused by cement injection.The efficiency of SF reducing the risk of cardiovascular complication was evaluated.[Method]Twenty-four pigs were assigned to 3 groups randomly,with 8 pigs in each group.After general anesthesia,animals were endotracheal intubated and mechanically ventilated by a respirator.The pulmonary trunk was exposed via sternotomy and each kind of cement was injected into the main pulmonary artery.Hemodynamic parameters were continuously monitored.Blood samples were drawn at certain time points for blood gases analyzing and measurement of AT Ⅲ.Postmortem lungs were subject to computer tomography(CT) examination along with three-dimensional reconstructions of cement casts.[Result]The injection of CPC caused severe pulmonary hypertension and arterial hypotension,with a maximum elevation of 20.93?5.04 mmHg in PAP(20 min post-injection) and a maximum decrease of-26.59?6.8 mmHg in MABP(50 min post-injection).The changes in SF/CPC group were relatively slighter,which is similar with PMMA group,having a statistically significant difference from CPC groups(P
