Determination of Arsenic Species in Urine by High Performance Liquid Chromatography and Hydrid Genesis Atomic Fluorescence Spectroscopy
- VernacularTitle:尿中砷的高效液相-氢化物发生原子荧光光谱测定法
- Author:
Jun WU
;
Shunhua WU
;
Yan MA
- Publication Type:Journal Article
- Keywords:
Chromatography,liquid;
Hydrid genesis atomic fluorescence spectroscopy;
Urine;
Arsenic species analysis
- From:
Journal of Environment and Health
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of high performance liquid chromatography and hydrid genesis atomic fluorescence spectroscopy(HPLC-HGAFS) about arsenic species in urine of rats treated with sodium arsenite.Methods The solution containing 15 mmol/L(NH4)2HPO4(pH=6.0)was used as the chromatographic eluant,the flow rate of which was 1.0 ml/min.The parameters of HPLC-HGAFS consisted of multiplier negative high voltage,hollow-cathode lamp's joint current and co-current,the flow rate of carrier gas,the flow rate of barrier shield gas,supporting liquid and reducing agent,which were 285 V,80 mA,36 mA,400 ml/min,600 ml/min,7%HCl and the mixed liquor of 1.5%KBH4 and 0.35% KOH respectively.Results The linear range of method was 20-100 ?g/L,and the linearity of regression equation of iAs3+,iAs5+,DMA was better.The lowest detection concentration of iAs3 +,iAs5 +,DMA in urine were 12.03,6.67 and 8.66 ?g/L respectively and all of the coefficient correlation were ≥0.98.The average recovery rates of DMA were 96.5%-103.4% and RSDs were 0.39%-1.26%.Conclusion The method has been proved accurate,sensitive and is applicable to the determination of arsenic species in urine.
