Effects of c-Jun N-terminal Kinase on Expression of Interleukin-10 in Keratinocytes Induced by Ultraviolet A
- VernacularTitle:c-jun氨基末端激酶在UVA诱导人角质形成细胞IL-10表达中的作用
- Author:
Li AN
;
Qian GAO
;
Guoqing DONG
- Publication Type:Journal Article
- Keywords:
Ultraviolet;
Keratinocyte;
Interleukin-10;
c-Jun N-terminal kinase;
SP600125
- From:
Journal of Environment and Health
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of c-Jun N-terminal kinase(JNK)on the expression of interleukin-10(IL-10) in keratinocytes induced by ultraviolet A(UVA).Methods The HaCaT cells in cultured were either sham irradiated(control) or exposured to 2.4 J/cm2 UVA radiation.The cells were collected at 0-48 h after irradiation,and JNK levels in cells were detected with the immunofluorescence.HaCaT cells were treated with SP600125(a JNK inhibitor) before irradiation,then cells and suspended medium were collected at each time-point after irradiation,and the expression of IL-10 mRNA and protein were determined by RT-PCR and ELISA.Results Compared with control cells,irradiated cells had increased levels of phospho-JNK throughout the entire 48 h following irradiation(P
