Effect of ischemic preconditioning on the apoptosis of hepatocytesand expression of HIF-1? and Survivin in rats
- VernacularTitle:缺血预处理对大鼠肝细胞凋亡及HIF-1?和Survivin蛋白表达的影响
- Author:
Kun WANG
;
Rong LI
- Publication Type:Journal Article
- Keywords:
Ischemic preconditioning;
Reperfusion injury;
Liver;
Apoptosis;
hypoxia-inducible factor-1?;
Survivin;
Rats,sprague-dawley
- From:Chinese Journal of Current Advances in General Surgery
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of ischemic preconditioning(IP)on hepatocellular apoptosis caused by ischemia-reperfus ion(IR)injury and expression of apoptosis regulatory genes(Survivin,HIF-1?)protein,to explore the protective mechanisms of IP on IR injury in rat liver.Methods:Ninty SD rats were randomly divided into sham operation(SO)group,ischemia-reperfusion(IR)group,ischemic preconditioning(IP)group.In SO group,the hepatoduodenal ligament of rat was only pulled and separated,and then closed the abdomen;IR group subjected to 30 minutes of ischemia;IP group was achieved by ischemia for 5 minutes,followed by reperfusion for 5 minutes before continuous to ischemia for 30 minutes.Ten rats of each group were killed after reperfusion for 2,6,12,24 hours respectively,then liver tissues were taken out.The rats in SO group were killed after operation for 2 hours.To detect the Apotosis index(A)Iand Survivin,HIF-1? protein levels.Results:AI of hepotocytes significantly increased in IR group and IP group than that in SO group(P
