HISTOCHEMICAL AND CYTOCHEMICAL OBSERVATIONS OF ACPase AND ATPase IN RAT LIVER AFTER ~(60)Co RADIATION
- VernacularTitle:~(60)Co照射后大鼠肝内酸性磷酸酶和腺苷三磷酸酶变化的组织化学和细胞化学观察
- Author:
Yu FENG
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Adult male rats weighing 250-300 g were used to study ACPase and ATPase in the liver following 60Co radiation on the whole body for a single dose 2000 rad(95.7-99r/min). The irradiated group and the control group were sacrificed at intervals of 1, 2, 3 days after irradiation. The left liver lobe was removed, fro/en in dryice-isopentane, and then cut in a cryostat. ACPase and ATPase activities were revealed by histochemical and cytochemical methods respectively.In the control group, ACPase activity was found to be mainly localized in the vicinity of bile canaliculi, and generally higher in the periportal region than in the centrilobular region. The distribution of ATPase reaction products was much like that of ACPase.On the 1st day after irradiation, a slight decrease was found in ACPase and ATPase activities, while on the 2nd and 3rd days in both of them was found a marked increase especially in the periportal region. These results were in agreement with cytochemical observations.The significance of these results and related problems were discussed.
