APPLIANCE OF IN VITRO ANTIBODY RESPONSE SYSTEM FOR STUDY OF IMMUNOREGULATORY EFFECTS OF THE TRADITIONAL CHINESE MEDICINALS AND SYNTHETIC CHEMICALS
- VernacularTitle:体外抗体应答系统用于研究中药及化学合成药的免疫调节效用
- Author:
Chianghua MENG
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
1983;0(S1):-
- CountryChina
- Language:Chinese
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Abstract:
Four kinds of commonly used injections and four refined components of the tranditional Chinese medicinals as well as one synthetic remedy, CMS or 404, have been studied for the immunoregulatory effects by using in vitro antibody cresponse system of Mishell and Button and by direct hemolytic plaque assay of Jerne. The results showed that among the injection remedies, 0.05-5% Baphica-canthes cusia, Brenk, and Leonurus heterophyllus, sweet, had immunosuppressive effects with little effect on cell viability, while 0.0005% of the former remedy showed immunopotentiation; 0.05-5% salvia miliorrhiza,Bge, had immunosuppresive effect with greater inhibitory effect on cell viability; Gentiana scabra, Bge, had weaker immunosuppressive effect (0.5-5%) with stronger inhibitory effect on cell viability. Among refined components, 0. 1?g/ml of Mangiferin showed immunosuppres-sion with no action on cell viability. Both Emodin monoglucoside and paeonol had weaker immunosuppressive effect (50-100?g/ml) and markedly affected cell viability; Acanthopanax senticosus polysaccharide (PES) showed immunosuppression in higher concentration (500?g/ml) and marked immunopotentiation at lower concentration (0.01-100?g/ml) with no effect on cell viability. A synthetic remedy, carboxymethylated starch (CMS or 404) had markedly immunopotentiating effect at 0.01-500?g/ml with no harmful effect on cell viability. The experiment revealed that in order to induce antibody in vitro, the adherent cells were necessary. Discussions have been made on the role of adherent cells in helping antibody response in vitro, and an evaluation on the advantages and disadvantages of the in vitro tests used for study of immunoregulatory drugs.
