Determination of Paraquat in Vegetables by High Performance Liquid Chromatography
- VernacularTitle:蔬菜中百草枯的高效液相色谱测定法
- Author:
Aiyue WANG
;
Zhilei ZHAI
- Publication Type:Journal Article
- Keywords:
Chromatography, liquid;
Vegetable;
Paraquat
- From:
Journal of Environment and Health
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of paraquat in vegetables by high performance liquid chromatography(HPLC). Methods The HPLC separation was conducted by using an Agilent TC-C18 column (4.6 mm?250 mm,5?m). The detection wavelength was 257 nm. The eluent is the mixture of 10 mmol/L sodium heptanesulfonate and methanol (50+50) with the flow rate of 1.0 ml/min. Results The linearity of the method ranged 0.00-10.0 ?g/ml. The concentration of paraquat showed a good linear relationship (R2=0.999 4), the RSDs were 0.75%-3.65%, the rates of recovery were 96.1%-104.0%, and the limit of detection was 0.03 ?g/ml. Conclusion This simple and accurate method is applicable to the determination of paraquat in vegetables.
