Expression of VEGF and its mechanism of invasion in gastric carcinoma
- VernacularTitle:VEGF在胃癌中的表达及其侵袭机制的研究
- Author:
Peilong LIAN
;
Rui ZHAO
;
Yanfeng LIU
;
Haizhang MA
- Publication Type:Journal Article
- Keywords:
Gastric neoplasm?Vascular endothelial growth factor?Matrix metalloproteinases-9
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of vascular endothelial growth factor (VEGF)and the significance of matrix metalloproteinases-9 (MMP-9), which is modulated by VEGF, in the invasion of gastric carcinoma. Methods: Expression of VEGF in gastric carcinoma and normal gastric tissues was detected by Western blot. MTT was used to measure the proliferation of AGS cells while real-time PCR and Western blot were used to measure the expression of MMP-9 at mRNA and protein level in AGS cells. Results: The expression of VEGF in gastric carcinoma was significantly higher than that of controls; the expression of MMP-9 in AGS cells with VEGF was significantly higher than that of controls at mRNA and protein level. Conclusion: Both VEGF and MMP-9 participate in the progression of gastric carcinoma, and VEGF may promote the invasion of gastric carcinoma by upregulating the expression of MMP-9.
